Identification of the plant acp3U tRNA modifying enzyme using primer extension in yeast

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dc.contributor.authorSebree, Nicholas
dc.date.accessioned2021-08-16T14:27:23Z
dc.date.available2021-08-16T14:27:23Z
dc.date.created2020
dc.description2020 Celebration of Student Research and Creativity presentationen_US
dc.description.abstracttRNA is required to synthesize proteins from mRNA, and posttranscriptional modifications are vital for making functional proteins. m2,2G, formed by TRM1, blocks base pairing, allowing for successful identification of the modification using fluorescent primer extension in yeast cells. This technique is being expanded to identify modifications in higher eukaryotes. Plant tRNALeu(AAG) and each of the predicted plant TRM1 genes have been individually transformed into trm1Δ yeast cells. Primer extension analysis demonstrated that two genes are responsible for the modification. Currently we are working to identify other posttranscriptional tRNA modifications that could have an impact on mental wellbeing of humans.en_US
dc.description.urihttps://youtu.be/_buYuQaYaxken_US
dc.identifier.urihttp://hdl.handle.net/11216/3922
dc.language.isoen_USen_US
dc.publisherNorthern Kentucky Universityen_US
dc.relation.ispartofseriesCelebration of Student Research and Creativity;2020
dc.subjectYeasten_US
dc.subjectTransfer RNAen_US
dc.subjectGenetic transcriptionen_US
dc.titleIdentification of the plant acp3U tRNA modifying enzyme using primer extension in yeasten_US
dc.typePresentationen_US

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