Determining Important Residues in THADA Responsible for the Methylation of Residue 32 in tRNAs

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Northern Kentucky University
Post-transcriptional modifications of tRNAs are important for their biological function. Modifications in or around the anticodon loop of tRNA aid in translation, while modifications in the body aid in its stability. Yeast Trm7 methyltransferase methylates residues 32 (C32) and 34 (N34) of tRNAs in yeast. Trm732 is a binding partner of Trm7 and must be bound for methylation of C32 to occur. THADA is the human ortholog of the yeast protein Trm732. THADA alleles are associated with type II diabetes and polycystic ovary syndrome. The goal of this research is to determine region(s) of THADA required for methylation of C32.
2020 Celebration of Student Research and Creativity presentation
Transfer RNA, Anticodone, Yeast, Proteins