The Impact of tRNA-Phe Anticodon Loop Modifications on Oxidative Stress Recovery in Saccharomyces cerevisiae

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dc.contributor.authorSeibert, Samuel
dc.contributor.authorEckart, Alexis
dc.contributor.authorGuy, Michael P.
dc.date.accessioned2021-12-08T15:14:05Z
dc.date.available2021-12-08T15:14:05Z
dc.date.created2021
dc.description2021 Celebration of Student Research and Creativity presentationen_US
dc.description.abstract"Transfer RNA anticodon loop modifications in the form of 2`-O methylations are critical for efficient translation in Saccharomyces cerevisiae (brewer’s yeast). The methyltransferase Trm7 associates with proteins Trm732 and Trm734 to methylate the thirty-second (C-32) and thirtyfourth (G- 34) nucleotides of tRNA- Phe, respectively. Yeast strains lacking either modification show healthy phenotypes, but sick growth is exhibited when both modifications are absent. Because oxidative stress necessitates efficient protein production, translational deficiencies may be magnified in trm732Δ and trm734Δ exposed to oxidative stress. It is established that trm7Δ mutants show lowered oxidative stress resistance, but the relative extent of trm732Δ and trm734Δ stress resistance is less understood. Spot tests confirm lowered stress resistance in trm7Δ mutants, but not in trm732Δ and trm734Δ mutants."en_US
dc.description.urihttps://youtu.be/LHdM1pzzO3Ien_US
dc.identifier.urihttp://hdl.handle.net/11216/4192
dc.language.isoen_USen_US
dc.publisherNorthern Kentucky Universityen_US
dc.relation.ispartofseriesCelebration of Student Research and Creativity;2021
dc.subjectTranser RNAen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectYeasten_US
dc.titleThe Impact of tRNA-Phe Anticodon Loop Modifications on Oxidative Stress Recovery in Saccharomyces cerevisiaeen_US
dc.typePresentationen_US

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