Browsing by Creator "Seibert, Samuel"
Now showing 1 - 4 of 4
Results Per Page
Sort Options
Item Anticodon Loop Modifications Enhance Oxidative Stress Recovery in Yeast(Northern Kentucky University) Seibert, Samuel; Guy, Michael P"Across eukaryotic organisms, 2`-O methylations on the anticodon loops of tRNA molecules are widely conserved and crucial for efficient translation. The methyltransferase Trm7 interacts with the protein Trm732 to methylate nucleotide C32 and interacts with the protein Trm734 to methylate nucleotide G34 on tRNA phenylalanine. Yeast cells lacking both modifications are sick, but the presence of either modification causes a healthy phenotype. Oxidative stress caused by free radicals stimulates metabolic responses which upregulate the transcription of stress recovery genes. Previous research indicates that trm7Δ mutants and trm734Δ mutants have lowered resistance to oxidative stress. In this study,trm732Δ, trm734Δ and trm7Δ mutants showed lowered, yet comparable levels of oxidative stress resistance."Item The Impact of tRNA-Phe Anticodon Loop Modifications on Oxidative Stress Recovery in Saccharomyces cerevisiae(Northern Kentucky University) Seibert, Samuel; Eckart, Alexis; Guy, Michael P."Transfer RNA anticodon loop modifications in the form of 2`-O methylations are critical for efficient translation in Saccharomyces cerevisiae (brewer’s yeast). The methyltransferase Trm7 associates with proteins Trm732 and Trm734 to methylate the thirty-second (C-32) and thirtyfourth (G- 34) nucleotides of tRNA- Phe, respectively. Yeast strains lacking either modification show healthy phenotypes, but sick growth is exhibited when both modifications are absent. Because oxidative stress necessitates efficient protein production, translational deficiencies may be magnified in trm732Δ and trm734Δ exposed to oxidative stress. It is established that trm7Δ mutants show lowered oxidative stress resistance, but the relative extent of trm732Δ and trm734Δ stress resistance is less understood. Spot tests confirm lowered stress resistance in trm7Δ mutants, but not in trm732Δ and trm734Δ mutants."Item Microwave Irradiation of Heteroaryl Compounds with Tropinone for the Synthesis of Aromatase Inhibitors(Northern Kentucky University) Ungar, Liat; Seibert, Samuel"Heteroaryl compounds are important molecules with extensive significance in medical chemistry. In our lab, we reacted heteroaryl compounds (4- Bromoquinolone) with tropinone using microwave irradiation at 160°C and 180°C in the presence of palladium catalyst. Techniques such as NMR, MPLC, and computer modeling were then utilized to isolate and purify the compound as well as assess its potential as an orally active drug. Overall, this research is significant because the product obtained could possibly serve as an aromatase inhibitor which would help in the treatment of breast cancer patients."Item NKU Course-Based Undergraduate Research Experience: The Identification of Potential Aromatase Inhibitors Based on Computer Modeling(Northern Kentucky University) Seibert, Samuel; Roy, Ayanav; Ungar, Liat"Microwave-assisted palladium catalyzed alpha- heteroarylation reactions were performed to join tropinone with heteroaryl halide compounds 4- bromoisoquinoline and 6- bromoquinoline to obtain potential aromatase inhibitors for the treatment of breast cancer. Computer modeling reveals that both target products show high enzyme-pocket docking scores compared to other tropinone-heteroaryl products synthesized in this course-based research lab. Herein, binding pocket interactions of various tropinone-derived compounds are compared to provide a rationale for further testing of these target products."