Browsing by Creator "Kyntchev, Angela"
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Item Assessing Adult Memory and Learning in Three Genotypes of Mice Exposed to Benzo[a]Pyrene During Early Brain Development(Northern Kentucky University) Patel, Shreyaben; Clough, Katelyn; Foster, Emma; Berling, Kevin; Honaker, Amanda; Kyntchev, Angela; Towell, Aria; Perry, Connor; Mullaguru, Jayasree; DeBurger, Emma; Jenkins, Kayla; Ferguson, Victoria; Forrest, Tyler; Niang, Diarra"Benzo-[a]-pyrene (BaP) is a polycyclic aromatic hydrocarbon and a major component of trafficrelated air pollution. It has been linked to learning and memory deficits. BaP binds to the aryl hydrocarbon receptor (AHR) and is metabolized by CYP1 enzymes. We look for genetic differences that affect susceptibility to early life BaP exposure using a mouse model and tests of learning and memory. BaP- treated AhrbCyp1a2(-/-) mice took significantly longer to find an escape platform in the Morris water maze. BaP- exposed mice also had significantly impaired reference memory in the maze probe trials and in the novel object test of visual recognition memory."Item Evaluating neonatal reflexes in mice exposed to benzo[a]pyrene during early brain development(Northern Kentucky University) Kyntchev, AngelaBenzo[a]pyrene (BaP) is a pollutant and a known carcinogen. Exposure from BaP can come from vehicle emissions, tobacco smoke, and grilled food. Pregnant mice were dosed with BaP or corn oil on small pieces of cereal from gestational day 10 to weaning at postnatal day 25. We tested neonatal reflexes using the righting reflex test at P5, 7 and 10. We also used the negative geotaxis test at P7, 10, and 14. We compared three genotypes of mice to determine if genetic differences affected susceptibility. We found that both treatment and genotype affected performance on the tests.Item Motor function changes in three genotypes of mice exposed to benzo[a]pyrene during gestation and lactation(Northern Kentucky University) Feltner, Mackenzie; Clough, Katie; Foster, Emma; Kyntchev, Angela; Towell, Aria; Patel, Shreyaben; Honaker, Amanda; Perry, Connor; Berling, Kevin"Benzo[a]pyrene (BaP) is a known carcinogen found in automobile exhaust, wood fire smoke, tobacco smoke, grilled food and coal tar. Many studies found exposure to these pollutants during pregnancy can damage genes and the placenta and impair fetal growth. We use mice that mimic human genetic variation in genes related to BaP metabolism to identify those at highest risk. In this study, we looked at motor function in adult mice exposed to BaP during gestation and lactation. We found no effect of genotype, but discovered Cyp1a2 knockout mice have deficits in motor coordination on the rotarod test."Item Synthesis of Heteroaryl Compounds Through Palladium Catalysis for Pharmaceutical Developments in a Course-based Undergraduate Research Experience(Northern Kentucky University) Kuennen, Kaylee; Kyntchev, Angela"At Northern Kentucky University, under the Undergraduate Research in Organic Chemistry Course (UROCC) curriculum and with the use of Course- based Undergraduate Research Experiences (CURE) labs, students are able to understand and experiment with the synthesis of heteroaryl compounds in a palladium catalyzed reaction for potential medicinal uses. The chemical techniques used in this lab include microwave reactor, medium pressure liquid chromatography (MPLC), nuclear magnetic resonance (NMR), infrared (IR), and the related digital tools. Our study provided some preliminary results for the initial stage of drug discovery for pharmaceutics against breast cancer."