Browsing by Creator "Perry, Connor"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
Item Assessing Adult Memory and Learning in Three Genotypes of Mice Exposed to Benzo[a]Pyrene During Early Brain Development(Northern Kentucky University) Patel, Shreyaben; Clough, Katelyn; Foster, Emma; Berling, Kevin; Honaker, Amanda; Kyntchev, Angela; Towell, Aria; Perry, Connor; Mullaguru, Jayasree; DeBurger, Emma; Jenkins, Kayla; Ferguson, Victoria; Forrest, Tyler; Niang, Diarra"Benzo-[a]-pyrene (BaP) is a polycyclic aromatic hydrocarbon and a major component of trafficrelated air pollution. It has been linked to learning and memory deficits. BaP binds to the aryl hydrocarbon receptor (AHR) and is metabolized by CYP1 enzymes. We look for genetic differences that affect susceptibility to early life BaP exposure using a mouse model and tests of learning and memory. BaP- treated AhrbCyp1a2(-/-) mice took significantly longer to find an escape platform in the Morris water maze. BaP- exposed mice also had significantly impaired reference memory in the maze probe trials and in the novel object test of visual recognition memory."Item Assessing Adult Motor Function in Three Genotypes of Mice Exposed to Benzo[a]Pyrene During Early Brain Development(Northern Kentucky University) Perry, Connor"Benzo[a]pyrene (BaP) is a carcinogenic polycyclic aromatic hydrocarbon commonly found in traffic- related air pollution, tobacco smoke, and grilled foods. BaP is linked to learning deficits and to neurodevelopmental delays in human and animal studies. We are using a mouse model to determine if genetic differences increase susceptibility to BaP exposure during early brain development. Mice lacking the CYP1A2 metabolic enzyme and wild type control mice were exposed to BaP from gestational day 10 (GD10) through weaning at postnatal day 25 (P25). A battery of motor function tests were performed when the mice reached young adulthood (P60). We used a pole climb test and rotarod to assess motor function and motor learning. There was a significant main effect of genotype with the time to turn (P < 0.001) and the total time to descend the pole (P < 0.01). In the rotarod test, there was also a main effect of genotype with AhrbCyp1a2(-/-) knockout mice showing impaired performance on Days 1, 2 and 5 of the test. This suggests that early life BaP exposure has minimal effect on motor function in adults."Item Motor function changes in three genotypes of mice exposed to benzo[a]pyrene during gestation and lactation(Northern Kentucky University) Feltner, Mackenzie; Clough, Katie; Foster, Emma; Kyntchev, Angela; Towell, Aria; Patel, Shreyaben; Honaker, Amanda; Perry, Connor; Berling, Kevin"Benzo[a]pyrene (BaP) is a known carcinogen found in automobile exhaust, wood fire smoke, tobacco smoke, grilled food and coal tar. Many studies found exposure to these pollutants during pregnancy can damage genes and the placenta and impair fetal growth. We use mice that mimic human genetic variation in genes related to BaP metabolism to identify those at highest risk. In this study, we looked at motor function in adult mice exposed to BaP during gestation and lactation. We found no effect of genotype, but discovered Cyp1a2 knockout mice have deficits in motor coordination on the rotarod test."