Browsing by Creator "Honaker, Amanda"
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Item Assessing Adult Memory and Learning in Three Genotypes of Mice Exposed to Benzo[a]Pyrene During Early Brain Development(Northern Kentucky University) Patel, Shreyaben; Clough, Katelyn; Foster, Emma; Berling, Kevin; Honaker, Amanda; Kyntchev, Angela; Towell, Aria; Perry, Connor; Mullaguru, Jayasree; DeBurger, Emma; Jenkins, Kayla; Ferguson, Victoria; Forrest, Tyler; Niang, Diarra"Benzo-[a]-pyrene (BaP) is a polycyclic aromatic hydrocarbon and a major component of trafficrelated air pollution. It has been linked to learning and memory deficits. BaP binds to the aryl hydrocarbon receptor (AHR) and is metabolized by CYP1 enzymes. We look for genetic differences that affect susceptibility to early life BaP exposure using a mouse model and tests of learning and memory. BaP- treated AhrbCyp1a2(-/-) mice took significantly longer to find an escape platform in the Morris water maze. BaP- exposed mice also had significantly impaired reference memory in the maze probe trials and in the novel object test of visual recognition memory."Item Assessing Neonatal Function in Three Genotypes of Mice Exposed to Benzo[a]pyrene(Northern Kentucky University) Honaker, Amanda"During Early Brain Development Benzo[a]pyrene (BaP) is a pollutant and a known carcinogen. Exposure from BaP can come from vehicle emissions, tobacco smoke, and grilled food. BaP exposure has been linked to learning deficits as well as delays in neurological development in both animals and humans. We used a mouse model to mimic the human genetic variation in the aryl hydrocarbon receptor and CYP1A2, which are genes related to BaP metabolism. Pregnant mice were dosed with BaP in corn oil soaked cereal or the corn oil vehicle from gestational day 10 to weaning at postnatal day 25 (P25). We tested neonatal reflexes using the righting reflex test at P5, 7 and 10. We also used the negative geotaxis test at P7, 10, and 14. We compared three genotypes of mice to determine if genetic differences affected susceptibility to BaP. We found that both treatment and genotype affected the mice’s’ reflex performance on the tests."Item Motor function changes in three genotypes of mice exposed to benzo[a]pyrene during gestation and lactation(Northern Kentucky University) Feltner, Mackenzie; Clough, Katie; Foster, Emma; Kyntchev, Angela; Towell, Aria; Patel, Shreyaben; Honaker, Amanda; Perry, Connor; Berling, Kevin"Benzo[a]pyrene (BaP) is a known carcinogen found in automobile exhaust, wood fire smoke, tobacco smoke, grilled food and coal tar. Many studies found exposure to these pollutants during pregnancy can damage genes and the placenta and impair fetal growth. We use mice that mimic human genetic variation in genes related to BaP metabolism to identify those at highest risk. In this study, we looked at motor function in adult mice exposed to BaP during gestation and lactation. We found no effect of genotype, but discovered Cyp1a2 knockout mice have deficits in motor coordination on the rotarod test."