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Investigation of the activity of tRNA modification enzymes Trm732 and Trm734

Show simple item record Sizemore, Hannah 2021-08-16T14:41:15Z 2021-08-16T14:41:15Z 2020
dc.description 2020 Celebration of Student Research and Creativity presentation en_US
dc.description.abstract Transfer RNAs (tRNAs) are molecules that transport amino acids to the ribosome to facilitate protein synthesis. Post-transcriptional modifications of tRNA are required for function. Trm7 is a methyltransferase enzyme which interacts with Trm732 and Trm734 to modify tRNAs at residues C32 and G34, respectively. Trm732 and Trm734 variants have been generated via site-directed mutagenesis and transformed into Saccharomyces cerevisiae. Analysis of these variants, when compared to wild type, allows for the identification of the residues involved in the methylation of tRNA. Function has been investigated quantitatively by measuring nucleoside modification levels with Ultra-Pressure Liquid Chromatography, and qualitatively through growth assays. en_US
dc.description.uri en_US
dc.language.iso en_US en_US
dc.publisher Northern Kentucky University en_US
dc.relation.ispartofseries Celebration of Student Research and Creativity;2020
dc.subject Transfer RNA en_US
dc.subject Genetic transformation en_US
dc.subject Enzymes en_US
dc.title Investigation of the activity of tRNA modification enzymes Trm732 and Trm734 en_US
dc.type Presentation en_US

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